宁静致远
吴超
Chao WU
系统与物理生物学研究所
特聘研究员
wuchao@szbl.ac.cn
Timeline
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2023 至今
深圳湾实验室 特聘研究员
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2016-2023
圣路易斯华盛顿大学 病理与免疫学系 博士后、讲师
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2011-2016
圣路易斯华盛顿大学 计算与分子生物物理学博士
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2008-2011
北京生命科学研究所 研究助理
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2005-2008
中国农业大学 生命科学荣誉学士
研究领域
应用生物化学、病毒学、人工智能等多学科研究工具，系统研究病毒复制及免疫逃逸机理，为开发基因递送工具奠定基础。包括1）病毒包涵体的发生机制以及体外生化重构，在病毒复制以及同宿主互作中的作用，致病机理及分子诊断。 2）利用前沿生物物理手段，开发对病毒颗粒进行基于形状及内容的分选技术，研究病毒颗粒形状对侵染及致病性的影响，为提升疫苗有效率提供理论基础。3）开发病毒复制小分子抑制剂以及高通量血清学技术。
成果
博士期间，利用核磁共振及细胞药物转运技术，对细菌多重耐药转运蛋白进行了系统的结构-动态-功能研究，为理解跨膜转运以及识别不同类型抗菌药物提供理论基础，为开发新型抗生素提供了思路。博后及讲师阶段于病理免疫系进行感染免疫方面的生化、结构、细胞、遗传学等多学科研究，对高致病埃博拉病毒、冠状病毒、人类偏肺病毒、合胞病毒的病毒复制机理及免疫逃逸机理进行了系统研究，并筛选了抗病毒小分子抑制剂。疫情期间，探索了新冠病毒N蛋白(核蛋白)作为检测标志物的独特性质，利用核蛋白不同片段，系统研究了血清学检测CTD结构域为特异性及灵敏性最佳标志物，合作开发了高通量血清学方法用于新冠抗体检测，为香港大规模血清学研究探讨mRNA及灭活疫苗的有效性半衰期提供了理论基础，对于新冠疫情期间的公共政策指导做出了重要贡献。相关工作发表在Annual Review of Virology, Cell, Nature Med., Nature Immunology, iScience, Respirology, ACS Infectious Diseases, Nature Communications, Antiviral Research, mBio 等期刊。埃博拉部分工作收录至最新版病毒学圣经Fields Virology丝状病毒章节。在Trends in Microbiology撰写关于埃博拉病毒复制起始机理的评论一篇。在Annual Review of Virology 撰写了病毒复制形成特殊细胞器的章节。 吴超博士现为美国病毒协会、微生物协会等行业协会成员，应邀为Nature Immunology, Nature Chemical Biology, mBio, Journal of Medical Virology 等行业期刊审稿。2021年遴选为eLife 期刊Early Career Reviewer, 2022年成为Vaccines 客座编辑及Topical Advisory Board成员，以及NIH COMPASS Scholar。2023年遴选为JBC期刊Early Career Reviewer，获得美国病毒学年会Global Scholar奖项。
荣誉
· Global Scholar Award from 42nd Annual Meeting of the American Society for Virology, 2023
· Early Career Reviewer for the Journal of Biological Chemistry, 2023
· Review Editor on the Editorial Board of Infectious Agents and Disease (specialty section of Frontiers in Medicine, Frontiers in Public Health and Frontiers in Microbiology) and on the Editorial Board of Virology (specialty section of Frontiers in Microbiology and Frontiers in Plant Science) 2023-
· Guest Editor and Topical Advisory board member for Vaccines 2022-
· NIH COMPASS Scholar 2022
· Member of Microbiology Society 2022-
· ASVCares Award from 41st Annual Meeting of the American Society for Virology, 2022
· Member of American Society for Virology 2022-
· Member of World Society for Virology 2022-
· Early-career reviewer for eLife 2021-
· Promoted talk and poster presentation prize in 6th Global Health & Infectious Disease Conference of Washington University in St. Louis, 2018
· Promoted talk in 14th Annual Postdoc Symposium of Washington University in St. Louis, 2018
· Poster prize in 22nd Annual Biochemistry and Computational & Molecular Biophysics Retreat of Washington University in St. Louis, 2015
· Student travel stipend and promoted talk in 55th Experimental Nuclear Magnetic Resonance Conference, 2014
· Poster Prize in National Institute of Biological Sciences 5th Annual Retreat, 2010
· Academic Excellence Award from China Agricultural University in 2006 and 2007
招聘信息
研究助理3-4名；联培博士研究生3-4名；博士后2-3名
https://mp.weixin.qq.com/s/5y-FHAD2jf3HqMjmGcKMig
代表论文
1. C. Wu*, N. Wagner, A. B. Moyle, A. Feng, N. Sharma, S. H. Stubbs, C. Donahue, R. Davey, M. L. Gross, D. W. Leung*, and G. K. Amarasinghe*. Disruption of Ebola NP0VP35 Inclusion Body-like Structures Reduce Viral Infection. Journal of Molecular Biology, 2023, Aug 18; 168241.
2. C. Wu, A. S. Holehouse, D. W. Leung, G. K. Amarasinghe, R. E. Dutch. Liquid phase partitioning in virus replication: observations and opportunities. Annual Review of Virology, 2022. 9:23.
3. A. J. Qavi, C. Wu, M. Lloyd, M. M.-U. Zaman, J. Luan, C. Ballman, D. W. Leung, S. L. Crick, G. K. Amarasinghe, and C. W. Farnsworth. Plasmonic Fluor-Enhanced Antigen Arrays for High-Throughput, Serological Studies of SARS-CoV-2. ACS Infectious Diseases. 2022, 8, 8.
4. Q. Ming, D. P. Celias, C. Wu, A. R. Cole, S. Singh, C. Mason, S. Dong, T. H. Tran, G. K. Amarasinghe, B. Ruffell, V. C. Luca. LAG3 ectodomain structure reveals functional interfaces for ligand and antibody recognition. Nature Immunology, 2022. 1-11.
5.C. Wu, A.J. Qavi, A. Hachim, N. Kavian, A.R. Cole, A.B. Moyle, N.D. Wagner, J. Sweeney-Gibbons, H.W. Rohrs, M.L. Gross, J.S.M. Peiris, C.F. Basler, C.W. Farnsworth, S.A. Valkenburg, G.K. Amarasinghe, D.W. Leung. Characterization of SARS-CoV-2 N protein reveal multiple functional consequences of the C-terminal domain. iScience. 2021, 24, 201681
6. W. Wang#, C. Wu#, G.K. Amarasinghe, D.W. Leung. Ebola virus replication stands out. Trends in Microbiology. 2019, 27(7), 565-566. (# co-first author)
7. C. Wu, S.A. Wynne, N.E. Thomas, E.-M. Uhlemann, C.G. Tate, K. Henzler-Wildman. Identification of an alternating-access dynamics mutant of EmrE with impaired transport. Journal of Molecular Biology. 2019, 431(15), 2777-2789.
8. Z. Su#, C. Wu#, L. Shi, P. Luthra, G.D. Pintilie, B Johnson, J.R. Porter, P. Ge, M. Chen, G. Liu, T. E. Frederick, J.M. Binning, G.R. Bowman, Z. Zhou, C. Basler, M.L. Gross, D.W. Leung, W. Chiu, and G.K. Amarasinghe. Electron cryo-microscopy structure of Ebola nucleoprotein reveals a mechanism for nucleocapsid-like assembly. Cell. 2018, 172: 1-13 (# co-first author)
9. N. Thomas, C. Wu, E. Morrison, A. Robinson, J. Werner, K. Henzler-Wildman. The C-terminus of bacterial multidrug transporter EmrE couples drug binding to proton release. Journal of Biological Chemistry. 2018, 293(49): 19137.
10. Y. Zhou, C. Wu, L. Zhao, N. Huang. Exploring the early stages of the pH-induced conformational change of influenza hemagglutinin. Proteins. 2014, 82: 2412-2428.
