Research Areas

Our research focuses on developing next-generation multi-dimensional biological imaging techniques and using them to explore the mystery of the complex biological system. Including:

1. multi-dimensional biological imaging techniques development;

2. the molecular mechanism of pathogen-host interactions;

3. Quantitative biomolecular dynamic imaging;

4. AI-assisted biological imaging.

Highlights

Dr. Hou Shangguo’s research focuses on the development of high spatiotemporal resolution optical microscopy techniques and their applications in biological systems. He has developed a series of multi-dimensional imaging methods to study the interactions between biomolecules and cells under various systems and conditions, addressing molecular and cellular systems at different scales. Shangguo’s work has been published in many high impact journals including Nature Communications, Science Bulletin, Small, Optics Letters, APL Photonics and Optics Express. The research work has been positively cited by prestigious journals such as Nature Photonics, Molecular Cell, and ACS Nano, and has been highlighted multiple times by academic websites such as Phys.org and Duke Research. He has been a member of American Biophysical Society and American Association for the Advancement of Science since 2016 and 2019, respectively.

Honors

2022: Guangdong province Talent - Top Young Talent Award

2021: Shenzhen high-level talent award

2020 Most Outstanding Postdoc Speaker of the Fitzpatrick Institute for Photonics, Duke University

2019: On being nominated for the 2019 Outstanding Postdoc at Duke University award

2015: Graduation with honor: Graduate Excellence Award of Shanghai

Related News

- Tracking Tiny Moving Targets 

- 3-D virus cam catches germs red-handed  

- Pushing the limits of microbiology: Tracking how viruses affect single cells

Selected Publications

1. Zhaoshuai Gao, Qian Li, Chunhai Fan*, Shangguo Hou*. Deciphering live-cell biomolecular dynamics with single-molecule fluorescence imaging. Science Bulletin. 2024. In Press. https://doi.org/10.1016/j.scib.2024.03.036

2. Hao Sha, Haoyang Li, Yongbing Zhang*, Shangguo Hou*. Deep learning-enhanced single-molecule spectrum imaging. APL Photonics, 2023, 8, 096102.

3. Xiufeng Zhang†, Haoyang Li†, Yue Ma†, Dongping Zhong*, and Shangguo Hou*. Study Liquid-liquid phase separation with optical microscopy: a methodology review. APL Bioengineering, 2023; 7(2): 021502. (Featured Article)

4. Shangguo Hou, Jack Exell, Kevin Welsher. Real-time 3D Single Molecule Tracking. Nature Communications, 2020, 11, 3607.

5. Shangguo Hou, Kevin Welsher. An Adaptive Real-Time 3D Single Particle Tracking Method for Monitoring Viral First Contacts. Small, 2019, 15, 1903039

6. Shangguo Hou, Xiaoqi Lang, Kevin Welsher. Robust real-time 3D single-particle tracking using a dynamically moving laser spot. Optics Letters, 2017, 42, 2390-2393.

7. Shangguo Hou†, Jianfang Chen†, Suhui Deng, Fei Wang, Qing Huang, Ya Cheng, Chunhai Fan. Improved resolution in fluorescence microscopy with the FRET pairs by time gating. Optics Express, 2015, 23, 13121-13129

8. Shangguo Hou, Le Liang, Suhui Deng, Jianfang Chen, Qing Huang, Ya Cheng, Chunhai Fan. Nanoprobes for super-resolution fluorescence imaging at the nanoscale. SCIENCE CHINA Chemistry, 2014, 57, 100-106.

9. Siddharth Patel, N Ashwanikumar, Ema Robinson, Yan Xia, Cosmin Mihai, Joseph Griffith III, Shangguo Hou, Adam Esposito, Tatiana Ketova, Kevin Welsher, John Joyal, Orn Almarsson, Gaurav Sahay. Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA. Nature Communications, 2020, 11, 983

10. Zhichao Liu, Ying Zhu, Liming Zhang, Weiping Jiang, Yawei Liu, Qiaowei Tang, Xiaoqing Cai, Jiang Li, Lihua Wang, Changlu Tao, Xianzhen Yin, Xiaowei Li, Shangguo Hou, Dawei Jiang, Kai Liu, Xin Zhou, Hongjie Zhang, Maili Liu, Chunhai Fan, Yang Tian. Structural and functional imaging of brains. SCIENCE CHINA Chemistry. 2023, 66, 324–36